Celery
SureFood® ALLERGEN Celery – NEW
Intended use:
The test detects celery DNA according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control.
For the quantitative determination the use of the laboratory reference material QUANTARD Allergen 40 containing 40 mg celery / kg food sample and a detection system including a standard dilution is required.
For DNA preparation, the use of SureFood® PREP Advanced, protocol 1, is recommended.
General Information:
The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, AriaDx and Agilent.
| Art. No. | S3605 |
|---|---|
| Test Format | 100 reactions* |
| Sample Preparation | 1. DNA preparation, e.g. SureFood® PREP ALLERGEN 2. Preparation of Allergen-PCR mix 3. Pipetting of samples and PCR mix 4. Check PCR protocol settings 5. Start PCR run |
| Detection Limit | Depending on the sample matrix, processing grade, DNA preparation and DNA contant, the detection is limited to < 5 DNA copies / ≤ 0.4 ppm. |
| Shelf life | 12 month |
| Additional Information | The detection system system can be used on all available real-time PCR cyclers.ABI Prism Eppendorf realplex iCycler Rotorgene LightCycler |
*Includes Internal Amplification Control (IAC) in VIC/HEX channel. SureFood®QUANTARD Allergen 40 must be used for quantifiction.
SureFood® ALLERGEN 4plex Soya/Celery/Mustard+IAC
Intended use:
Soya, Celery and Mustard detection by real-time PCR in one run.
The multiplex test detects DNA of soya, celery and mustard according to directive (EC) 1169/2011.
For DNA-preparation the use of SureFood® PREP Advanced, protocol 1 is recommended.
General Information:
The real-time PCR assay can be used with established real-time PCR instruments, equipped for the detection of four fluorescence emissions at 510 nm, 580 nm, 610 nm and 660 nm (FAM, VIC/HEX, ROX and Cy5) at the same time. The technical validation was performed on Agilent Mx3005P, Roche LightCycler® 480 II*, Applied Biosystems 7500, BioRad CFX96 und Qiagen Rotor-Gene Q.
| Art. No. | S3401 |
|---|---|
| Test Format | 100 reactions |
| Sample Preparation | 1. DNA preparation, e.g. SureFood® PREP ALLERGEN 2. Preparation of Allergen-PCR mix 3. Pipetting of samples and PCR mix 4. Check PCR protocol settings 5. Start PCR run |
| Detection Limit | Depending on the sample matrix, processing grade, DNA preparation and DNA contant, the detection is limited to < 5 DNA copies / ≤ 0.4 ppm. |
| Shelf life | 12 month |
| Additional Information | The detection system system can be used on all available real-time PCR cyclers.ABI Prism Eppendorf realplex iCycler Rotorgene LightCycler |
Allergens
Accessories
DNA Preparation
Almond
Brazil nut
Casein
Cashew kernel
Celery
Coconut
Crustaceans
Egg
Fish
Gliadin / Gluten
Hazelnut
Histamine
Lupine
Lysozyme
Macadamia nut
Milk
Molluscs
Mustard
Peanut
Pistachio