Peanut
Lateral Flow Peanut
Intended use:
The Lateral Flow Peanut assay is a qualitative detection assay of Peanut residues in surface samples got from e.g. production lines. The assay is also applicable for food samples. The assay is applied for the detection of small amounts of Peanut contaminations.
General Information:
Peanut contaminations in food possibly elicit hypersensitivity in sensitive persons. Food production lines have to be free of residues to prevent these allergic reactions. The effectiveness of cleaning can be verified by using the Lateral Flow Peanut Swabbing method.
| Art. No. | BL606-10 BL606-25 |
|---|---|
| Test Format | 10 x test strips (1 determination per strip) 25 x test strips (1 determination per strip) |
| Sample Preparation | Swabbing method: cleaning surface with pre-treated swab, wash out the swab and measure Food assay: homogenise food sample, filter, measure |
| Incubation Time | 10 min |
| Detection Limit | Swabbing method: 10 µg dried sample on surface Food assay (zero matrix): 1 ppm |
| External Link I | http://www.bioavid.de |
RIDASCREEN®FAST Peanut
Intended use:
RIDASCREEN®FAST Peanut test is a sandwich enzyme immunoassay for the quantitative analysis of peanuts or parts of peanuts in food. Peanut can be present as an ingredient or as a contamination in raw or heated food.
Breakfast cereals, cookies, ice cream and milk chocolate are approved by AOAC Research institute according to the Performance Tested Method Program with the license no. 030404.
General Information:
Even small traces of peanut in foodstuffs can trigger allergic reactions in sensitive individuals. In many countries peanut has to be declared on the food packaging according to the food allergen legislation.
RIDASCREEN®FAST Peanut was validated with breakfast cereals, cookies, ice cream and milk chocolate in an independent study by three independent US laboratories under the supervision of AOAC Research Institute. Each laboratory analyzed 20 blank samples and 20 samples spiked at 5 mg/kg peanut of each matrix. Two of 240 blank samples were measured positive (> 2.5 mg/kg peanut) corresponding to a specificity of 99.2 % (238/240). All of the spiked samples (240/240) were found positive (sensitivity = 100 %).
| Art. No. | R6202 |
|---|---|
| Test Format | Microtiter plate with 48 wells (6 strips with 8 removable wells each) |
| Standard Range | 0 ppm (zero standard), 2.5 ppm, 5 ppm, 10 ppm, 20 ppm peanut extractThe RIDASCREEN® standard material is calibrated to the reference material NIST SRM 2387 peanut butter. |
| Sample Preparation | homogenization, extraction and centrifugation |
| Incubation Time | 30 min |
| Detection Limit | 0.13 mg/kg (ppm) peanut corresponding to 0.00017 % peanut (0.0 – 0.2 depending on the matrix)
Samples tested negative still could contain a peanut contamination below the limit of detection of the assay, or they might contain other peanut components like lipids for example. |
| Cross Reactivity | Different kinds of nuts like hazelnut, almond, walnut, cashew, pistachio and pecan show no cross reaction. The cross reactivity to chickpea is 0.2 % and to green pea 0.001 %. |
| Specificity | The polyclonal antibody specifically detects peanut proteins, including the peanut allergen Ara h1 and Ara h2. Extracts from oat, corn, wheat, barley, sesame or soy do not interfere with the test. |
SureFood® ALLERGEN Peanut – NEW
Intended use:
The test detects peanut DNA according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control.
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 containing 40 mg peanut / kg food sample and a detection system including a standard dilution is required.
For DNA preparation, the use of SureFood® PREP Advanced, protocol 1, is recommended.
General Information:
The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, AriaDx and Agilent.
| Art. No. | S3603 |
|---|---|
| Test Format | Test-Kit with 100* reactions |
| Sample Preparation | 1. DNA preparation, e.g. SureFood® PREP ALLERGEN 2. Preparation of Allergen-PCR mix 3. Pipetting of samples and PCR mix 4. Check PCR protocol settings 5. Start PCR run |
| Detection Limit | Depending on the sample matrix, processing grade, DNA preparation and DNA contant, the detection is limited to ≤ 5 DNA copies / ≤ 1 ppm. |
| Shelf life | 12 month |
| Additional Information | The detection system system can be used on all available real-time PCR cyclers.ABI Prism Eppendorf realplex iCycler Rotorgene LightCycler |
*Includes Internal Amplification Control (IAC) in VIC/HEX channel. SureFood®QUANTARD Allergen 40 must be used for quantifiction.
SureFood® ALLERGEN 4plex Peanut/Hazelnut/Walnut+IAC
Intended use:
This multiplex test detects specific DNA sequences of peanut, hazelnut and walnut.
For DNA-preparation the use of SureFood® PREP Advanced, protocol 1 is recommended.
General Information:
The real-time PCR assay can be used with established real-time PCR instruments, equipped for the detection of four fluorescence emissions at 510 nm, 580 nm, 610 nm and 660 nm (FAM, VIC/HEX, ROX and Cy5) at the same time (Agilent MxSeries, Roche LightCycler® 480 , ABI PRISM, BioRad CFX96 etc.).
| Art. No. | S3402 |
|---|---|
| Test Format | 100 reactions |
| Sample Preparation | 1. DNA preparation, e.g. SureFood® PREP ALLERGEN 2. Preparation of Allergen-PCR mix 3. Pipetting of samples and PCR mix 4. Check PCR protocol settings 5. Start PCR run |
| Detection Limit | Depending on the sample matrix, processing grade, DNA preparation and DNA contant, the detection is limited to < 5 DNA copies / ≤ 1 ppm. |
| Shelf life | 12 month |
| Additional Information | The detection system system can be used on all available real-time PCR cyclers.ABI Prism Eppendorf realplex iCycler Rotorgene LightCycler |
Allergens
Accessories
DNA Preparation
Almond
Brazil nut
Casein
Cashew kernel
Celery
Coconut
Crustaceans
Egg
Fish
Gliadin / Gluten
Hazelnut
Histamine
Lupine
Lysozyme
Macadamia nut
Milk
Molluscs
Mustard
Peanut
Pistachio