Mustard
Lateral Flow Senf / Mustard
Intended use:
The Lateral Flow Mustard assay is a qualitative detection assay of Mustard residues in surface samples got from e.g. production lines. The assay is also applicable for food samples. The assay is applied for the detection of small amounts of Mustard contaminations.
General Information:
Mustard contaminations in food possibly elicit hypersensitivity in sensitive persons. Food production lines have to be free of residues to prevent these allergic reactions. The effectiveness of cleaning can be verified by using the Lateral Flow Mustard Swabbing method.
| Art. No. | BL603-10 BL603-25 |
|---|---|
| Test Format | 10 x test strips (1 determination per strip) 25 x test strips (1 determination per strip) |
| Sample Preparation | Swabbing method: cleaning surface with pre-treated swab, wash out the swab and measure Food assay: homogenise food sample, filter, measure |
| Incubation Time | 10 min |
| Detection Limit | Swabbing method: 10 µg dried sample on surface Food assay (zero matrix): 1 ppm |
| Cross Reactivity | rapeseed and seeds of other brassicaceae |
| External Link I | http://www.bioavid.de/ |
RIDASCREEN®FAST Senf / Mustard
Intended use:
RIDASCREEN®FAST Senf/Mustard is a sandwich enzyme immunoassay for the quantitative analysis of mustard in spices, meat and sausages, oils, mayonnaise and other food.
General Information:
Mustard is a popular spice that contains mustard oil, glycosides and more than 20 % proteins. These proteins can trigger allergies and must therefore be declared in many countries. Mustard allergens are in most cases heat resistant and relatively stable against an enzymatic treatment. Mustard can be present in many foods due to a cross contamination in the production process (especially if spices have been used in the product).
| Art. No. | R6152 |
|---|---|
| Test Format | Microtiter plate with 48 wells (6 strips with 8 removable wells each) |
| Standard Range | 0 ppm (zero standard), 0.5 ppm, 1.5 ppm, 4.5 ppm, 13.5 ppm mustard powder |
| Incubation Time | 30 min |
| Detection Limit | 0.1 mg/kg (ppm) mustard powder (0.08 – 0.11 depending on the matrix) Limit of quantification: 0.5 ppm mustard powder |
| Cross Reactivity | The antibodies used in the test specifically detect all kinds of mustard (yellow, white, brown, black mustard). Cross reactivity to rapeseed (67%) and other seeds of brassicaceae. |
SureFood® ALLERGEN Mustard – NEW
Intended use:
The test detects DNA of brown (Indian), yellow and black mustard according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control.
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 containing 40 mg mustard / kg food sample and a detection system including a standard dilution is required.
For DNA preparation, the use of SureFood® PREP Advanced, protocol 1, is recommended.
General Information:
The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, AriaDx and Agilent Mx3005P.
| Art. No. | S3609 |
|---|---|
| Test Format | Test-Kit with 100* reactions |
| Sample Preparation | 1. DNA preparation (SureFood® PREP Allergen, S1012) 2. PCR set up 3. PCR run |
| Detection Limit | < 5 DNA copies / 5≤ 0.4 ppm ppm depending on the sample matrix, processing grade, DNA preparation and DNA content. |
| Shelf life | 12 month |
| Additional Information | The detection system system can be used on all available real-time PCR cyclers.ABI Prism Eppendorf realplex iCycler Rotorgene LightCycler |
*Includes Internal Amplification Control (IAC) in VIC/HEX channel. SureFood®QUANTARD Allergen 40 must be used for quantifiction.
SureFood® ALLERGEN 4plex Soya/Celery/Mustard+IAC
Intended use:
Soya, Celery and Mustard detection by real-time PCR in one run.
The multiplex test detects DNA of soya, celery and mustard according to directive (EC) 1169/2011.
For DNA-preparation the use of SureFood® PREP Advanced, protocol 1 is recommended.
General Information:
The real-time PCR assay can be used with established real-time PCR instruments, equipped for the detection of four fluorescence emissions at 510 nm, 580 nm, 610 nm and 660 nm (FAM, VIC/HEX, ROX and Cy5) at the same time. The technical validation was performed on Agilent Mx3005P, Roche LightCycler® 480 II*, Applied Biosystems 7500, BioRad CFX96 und Qiagen Rotor-Gene Q.
| Art. No. | S3401 |
|---|---|
| Test Format | 100 reactions |
| Sample Preparation | 1. DNA preparation, e.g. SureFood® PREP ALLERGEN 2. Preparation of Allergen-PCR mix 3. Pipetting of samples and PCR mix 4. Check PCR protocol settings 5. Start PCR run |
| Detection Limit | Depending on the sample matrix, processing grade, DNA preparation and DNA contant, the detection is limited to < 5 DNA copies / ≤ 0.4 ppm. |
| Shelf life | 12 month |
| Additional Information | The detection system system can be used on all available real-time PCR cyclers.ABI Prism Eppendorf realplex iCycler Rotorgene LightCycler |
*Includes Internal Amplification Control (IAC) in VIC/HEX channel. SureFood®QUANTARD Allergen 40 must be used for quantifiction.
Allergens
Accessories
DNA Preparation
Almond
Brazil nut
Casein
Cashew kernel
Celery
Coconut
Crustaceans
Egg
Fish
Gliadin / Gluten
Hazelnut
Histamine
Lupine
Lysozyme
Macadamia nut
Milk
Molluscs
Mustard
Peanut
Pistachio