Lateral Flow Almond
The Lateral Flow Almond assay is a qualitative detection assay of Almond residues in surface samples got from e.g. production lines. The assay is also applicable for food samples. The assay should be used for the detection of small amounts of Almond contaminations.
Almond contaminations in food possibly elicit hypersensitivity in sensitive persons. Food production lines have to be free of residues to prevent these allergic reactions. The effectiveness of cleaning can be verified by using the Lateral Flow Almond Swabbing method.
|Test Format||10 x test strips (1 determination per strip)
25 x test strips (1 determination per strip)
|Sample Preparation||Swabbing method: cleaning surface with pretreated swab, wash out the swab and measure
Food assay: homogenise food sample, filter, measure
|Incubation Time||10 min|
|Detection Limit||Swabbing method: 10µg dried sample on surface
Food assay (zero matrix): 1 ppm
|Cross Reactivity||Apricot Seed: 100%Food assay: wheat flour|
RIDASCREEN®FAST Mandel / Almond
RIDASCREEN®FAST Mandel / Almond is a sandwich enzyme immunoassay for the quantitative analysis of almond or contamination of almond in food (ingredient components or contamination in raw and cooked food, like breakfast cereals, cookies, ice cream and chocolate).
Almond (Amygdalys communis) is a very aromatic fruit and one of the popular tree nuts worldwide. Almonds are components of a lot of snacks and breakfast cereals. As ingredients almonds occur in a lot of foods especially in products of the industry for cookies and chocolate.
Almonds belong to the subfamily of Prunoideae and are closely related to the peach and apricots. The high protein content of approx. 25 % is not only nutritious but also an allergic potential. Approximatly 95 % of the proteins are water soluble. Even consumption of few milligrams of almond can induce allergic reactions in highly allergic persons. Symptoms range from allergic skin reactions up to anaphylactic shock. Since almond allergy is persistent during life and treatment of this allergy is not possible, avoiding almonds is extremely important for these patients.
|Test Format||Microtiter plate with 48 wells (6 strips with 8 removable wells each)|
|Standard Range||0 ppm (zero standard), 2.5 ppm, 5 ppm, 10 ppm, 20 ppm almond|
|Sample Preparation||homogenization, extraction and centrifugation|
|Incubation Time||30 min|
|Detection Limit||0.1 mg/kg (ppm) almond corresponding to 0.00017 % almond (0 – 0.2 depending on the matrix)|
|Cross Reactivity||The polyclonal antibody specifically detects proteins from almonds. Cross reaction to apricot stone is > 100 %. No cross-reaction with cashew nut, brazil nut, pecan, hazelnut, coconut, macadamia nut, walnut, chest nut, sunflower seeds, sesame and lima beans.|
SureFood® ALLERGEN Almond – NEW
The test detects almond DNA according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control.
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 containing 40 mg almond / kg food sample and a detection system including a standard dilution is required.
For DNA preparation, the use of SureFood® PREP Advanced, protocol 1, is recommended.
The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, AriaDx and Agilent.
|Test Format||Test-Kit with 100* reactions|
|Sample Preparation||1. DNA preparation, e.g. SureFood® PREP ALLERGEN 2. Preparation of Allergen-PCR mix 3. Pipetting of samples and PCR mix 4. Check PCR protocol settings 5. Start PCR run|
|Detection Limit||Depending on the sample matrix, processing grade, DNA preparation and DNA contant, the detection is limited to < 5 DNA copies / ≤ 4 ppm.|
|Shelf life||12 month|
|Additional Information||The detection system system can be used on all available real-time PCR cyclers.ABI Prism
*Includes Internal Amplification Control (IAC) in VIC/HEX channel. SureFood®QUANTARD Allergen 40 must be used for quantifiction.
Gliadin / Gluten