RIDASCREEN®FAST Casein is a sandwich enzyme immunoassay for the quantitative analysis of casein in food like ice cream, wine, chocolate, beverages, infant formula, bakery goods, sausages, cake and bread mix.
Cow`s milk contains 3.2 % proteins which consist of 10 % ß-lactoglobulin (leading protein of whey) and 80 % caseins. The most important allergen is ß-lactoglobulin (especially for children) while the caseins become to be dominant later in adults.
Casein (lat. Caseus = cheese) is a rough flaked curdling protein, which forms micells in the milk and precipitates under acidic conditions. The group of caseins consists of the αs-caseins, the ß-caseins, the κ-casein, and the γ-caseins (proteolytic protein fragment of ß-casein by the milk protease plasmin). κ-casein is cleaved into a hydrophobic (para κ-casein) and into a water soluble polar component (macropeptid) by proteolysis, e.g. by using lab ferment.
Milk can be present as an ingredient or as a contamination in raw and processed food products. Milk must be declared as an ingredient on food labels as it can induce allergic reactions at infancy. Whey (ß-lactoglobulin) or caseinates (e.g. in sausages) are often added to food products, therefore it is recommended to determine casein in food.
|Test Format||Microtiter plate with 48 wells (6 strips with 8 removable wells each)|
|Standard Range||0 ppm (zero standard), 0.5 ppm, 1.5 ppm, 4.5 ppm,13.5 ppm casein|
|Sample Preparation||homogenization, extraction and centrifugation|
|Incubation Time||30 min|
|Detection Limit||Limit of detection (ice cream, wine, chocolate, beverages): 0.24 mg/kg (ppm) casein
Limit of detection (bakery goods, sausages, cake and bread mix): 1.36 mg/kg (ppm) casein
Limit of quantification (ice cream, wine, chocolate, beverages): 0.5 mg/kg (ppm)
Limit of quantification (bakery goods, sausages, cake and bread mix): 2.5 mg/kg (ppm)
|Cross Reactivity||The antibodies specifically detect α-, β- and κ-caseins of cow’s, sheep’s, goat’s and buffalo’s milk. No cross reactivity to β-lactoglobulin exists.|
|External Link I||http://www.bioavid.de/|
RIDA® Extractor 2
The RIDA® Extractor 2 (R4613) is used for the sample preparation in
- RIDASCREEN®FAST Milk (R4652)
- RIDASCREEN®FAST Casein (R4612)
- RIDASCREEN®FAST β-Lactoglobulin (R4902).
The sample preparation is described in the product information of the respective kits.
|Test Format||30 ml concentrate
sufficient for 15 samples
Gliadin / Gluten