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Casein

RIDASCREEN®FAST Casein

 

Intended use:

RIDASCREEN®FAST Casein is a sandwich enzyme immunoassay for the quantitative analysis of casein in food like ice cream, wine, chocolate, beverages, infant formula, bakery goods, sausages, cake and bread mix.

General Information:

Cow`s milk contains 3.2 % proteins which consist of 10 % ß-lactoglobulin (leading protein of whey) and 80 % caseins. The most important allergen is ß-lactoglobulin (especially for children) while the caseins become to be dominant later in adults.
Casein (lat. Caseus = cheese) is a rough flaked curdling protein, which forms micells in the milk and precipitates under acidic conditions. The group of caseins consists of the αs-caseins, the ß-caseins, the κ-casein, and the γ-caseins (proteolytic protein fragment of ß-casein by the milk protease plasmin). κ-casein is cleaved into a hydrophobic (para κ-casein) and into a water soluble polar component (macropeptid) by proteolysis, e.g. by using lab ferment.
Milk can be present as an ingredient or as a contamination in raw and processed food products. Milk must be declared as an ingredient on food labels as it can induce allergic reactions at infancy. Whey (ß-lactoglobulin) or caseinates (e.g. in sausages) are often added to food products, therefore it is recommended to determine casein in food.

Specifications
Art. No. R4612
Test Format Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Standard Range 0 ppm (zero standard), 0.5 ppm, 1.5 ppm, 4.5 ppm,13.5 ppm casein
Sample Preparation homogenization, extraction and centrifugation
Incubation Time 30 min
Detection Limit Limit of detection (ice cream, wine, chocolate, beverages): 0.24 mg/kg (ppm) casein
Limit of detection (bakery goods, sausages, cake and bread mix): 1.36 mg/kg (ppm) casein
Limit of quantification (ice cream, wine, chocolate, beverages): 0.5 mg/kg (ppm)
Limit of quantification (bakery goods, sausages, cake and bread mix): 2.5 mg/kg (ppm)
Cross Reactivity The antibodies specifically detect α-, β- and κ-caseins of cow’s, sheep’s, goat’s and buffalo’s milk. No cross reactivity to β-lactoglobulin exists.
External Link I http://www.bioavid.de/

RIDA® Extractor 2

 

Intended use:

The RIDA® Extractor 2 (R4613) is used for the sample preparation in

  • RIDASCREEN®FAST Milk (R4652)
  • RIDASCREEN®FAST Casein (R4612)
  • RIDASCREEN®FAST β-Lactoglobulin (R4902).

The sample preparation is described in the product information of the respective kits.

Specifications
Art. No. R4613
Test Format 30 ml concentrate
sufficient for 15 samples

 

Allergens

Accessories
DNA Preparation
Almond
Brazil nut
Casein
Cashew kernel
Celery
Coconut
Crustaceans
Egg
Fish
Gliadin / Gluten
Hazelnut
Histamine
Lupine
Lysozyme
Macadamia nut
Milk
Molluscs
Mustard
Peanut
Pistachio