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Hazelnut

Lateral Flow Hazelnut

 

Intended use:

The Lateral Flow Hazelnut assay is a qualitative detection assay of Hazelnut residues in surface samples got from e.g. production lines. The assay is also applicable for food samples. The assay is applied for the detection of small amounts of Hazelnut contaminations.

General Information:

Hazelnut contaminations in food possibly elicit hypersensitivity in sensitive persons. Food production lines have to be free of residues to prevent these allergic reactions. The effectiveness of cleaning can be verified by using the Lateral Flow Hazelnut Swabbing method.

Specifications
Art. No. BL604-10
BL604-25
Test Format 10 x test strips (1 determination per strip)
25 x test strips (1 determination per strip)
Sample Preparation Swabbing method: cleaning surface with pre-treated swab, wash out the swab and measure
Food assay: homogenise food sample, filter, measure
Incubation Time 10 min
Detection Limit Swabbing method: 10 µg dried sample on surface
Food assay (zero matrix): 1 ppm
Cross Reactivity Walnut: 0.1%, Pumpkin seed: 0.01 %
External Link I http://www.bioavid.de/

RIDASCREEN® FAST Hazelnut

 

Intended use:

RIDASCREEN®FAST Hazelnut is a sandwich enzyme immunoassay for the quantitative analysis of hazelnut and pieces of hazelnut in food such as cereals, baked goods, ice cream and chocolate.

The ELISA has been validated for dark chocolate by the German BVL (§64 method LMBG). The RIDASCREEN®FAST Hazelnut test is calibrated to the chocolate samples used in the German ring trial.

The published DIN CEN/TS 15633-2:2013 characterizes the ELISA method for the detection of hazelnut in food. The RIDASCREEN®FAST Hazelnut (R6802) by R-Biopharm fulfills the requirements of this DIN standard.

General Information:

Hazelnut can be present as an ingredient or as a contamination in raw and cooked products. According to the EU Directive 2007/68/EG from 27th November 2007, hazelnut must be declared on food labels as it can induce allergic reactions.

Specifications
Art. No. R6802
Test Format Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Standard Range 0 ppm (zero standard), 2.5 ppm, 5 ppm, 10 ppm, 20 ppm hazelnut
Sample Preparation homogenization, extraction and centrifugation
Incubation Time 30 min
Detection Limit 0.19 mg/kg (ppm) hazelnut corresponding to 0.00015 % hazelnut (0.17 – 0.22 depending on the matrix)
Cross Reactivity sun flower seeds: ≤ 0.07%linseeds (ground), almonds: not detectableThe detection depends on the degree of roasting. Highly roasted hazelnuts are recovered with approx. 80 %. The hazelnut extract used as a standard in this assay contains approx. 9 % protein.

SureFood® ALLERGEN Hazelnut- NEW

 

Intended use:

The test detects hazelnut DNA according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control.

For the quantitative determination the use of the laboratory reference materialSureFood® QUANTARD Allergen 40 containing 40 mg hazelnut / kg food sample and a detection system including a standard dilution is required.

For DNA preparation, the use of SureFood® PREP Advanced, protocol 1, is recommended.

General Information:

The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, AriaDx and Agilent.

Specifications
Art. No. S3602
Test Format Test-Kit with 100* reactions
Sample Preparation 1. DNA preparation, e.g. SureFood® PREP ALLERGEN 2. Preparation of Allergen-PCR mix 3. Pipetting of samples and PCR mix 4. Check PCR protocol settings 5. Start PCR run
Detection Limit Depending on the sample matrix, processing grade, DNA preparation and DNA contant, the detection is limited to ≤ 5 DNA copies / ≤ 0.4 ppm.
Shelf life 12 month
Additional Information The detection system can be used on all available real-time PCR cyclers.ABI Prism
Eppendorf realplex
iCycler
Rotorgene
LightCycler

*Includes Internal Amplification Control (IAC) in VIC/HEX channel. SureFood®QUANTARD Allergen 40 must be used for quantifiction.

 

SureFood® ALLERGEN 4plex Peanut/Hazelnut/Walnut+IAC

 

Intended use:

This multiplex test detects specific DNA sequences of peanut, hazelnut and walnut.

For DNA-preparation the use of SureFood® PREP Advanced, protocol 1 is recommended.

General Information:

The real-time PCR assay can be used with established real-time PCR instruments, equipped for the detection of four fluorescence emissions at 510 nm, 580 nm, 610 nm and 660 nm (FAM, VIC/HEX, ROX and Cy5) at the same time (Agilent MxSeries, Roche LightCycler® 480 , ABI PRISM, BioRad CFX96 etc.).

Specifications
Art. No. S3402
Test Format 100 reactions
Sample Preparation 1. DNA preparation, e.g. SureFood® PREP ALLERGEN 2. Preparation of Allergen-PCR mix 3. Pipetting of samples and PCR mix 4. Check PCR protocol settings 5. Start PCR run
Detection Limit Depending on the sample matrix, processing grade, DNA preparation and DNA contant, the detection is limited to < 5 DNA copies / ≤ 1 ppm.
Shelf life 12 month
Additional Information The detection system system can be used on all available real-time PCR cyclers.ABI Prism
Eppendorf realplex
iCycler
Rotorgene
LightCycler

Allergens

Accessories
DNA Preparation
Almond
Brazil nut
Casein
Cashew kernel
Celery
Coconut
Crustaceans
Egg
Fish
Gliadin / Gluten
Hazelnut
Histamine
Lupine
Lysozyme
Macadamia nut
Milk
Molluscs
Mustard
Peanut
Pistachio