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Histamine

 

 

RIDASCREEN® Histamine (enzymatic)

 

Intended use:

RIDASCREEN® Histamine (enzymatic) is used for the quantitative determination of histamine in fresh fish, canned fish, fish meal, wine* and cheese and milk.

*  For the sample preparetion of wine it is recommendedto use RIDA® Sample Decolorant (R1699)

Specifications
Art. No. R1605
Test Format Enzymatic test in microtiter plate format with 96 wells (12 strips with 8 removable wells each)
Standard Range 0 ppb, 0.5 ppb, 1.5 ppb, 5 ppb, 15 ppb, 50 ppb histamine
Sample Preparation different depending on the sample
Incubation Time 15 min incubation time
Detection Limit 0.75 – 3.75 mg/kg (ppm) histamine depending on the matrix
Cross Reactivity Histamine: 100 %3-Methyl-Histamine: ca. 0,01 %Tyramine: n.n.L-Phenylalanine: n.n.

L-Histidine: n.n.

L-Tyrosine: n.n.

Tryptamine: n.n.

5-Hydroxy-Indole-Acetic Acid: n.n.

Serotonine: n.n.

RIDASCREEN® Histamine

Intended use:

RIDASCREEN® Histamine is a competitive enzyme immunoassay for the quantitative analysis of histamine in white and red wine, sparkling wine, milk, cheese, fresh fish, canned fish and fish meal.

General Information:

Histamine is a product of the decomposition of histidine caused by the growth of certain bacteria in protein rich food like fish, cheese, meat as well as sparkling wine, wine and beer. The amount of histamine formed depends on the bacterial species, the temperature and time of exposure, and may exceed 1000 ppm (mg/kg). Fish containing high levels of histamine have been associated with poisoning commonly referred to as “scombroid poisoning”. Quality control measures have to be taken to minimize the occurrence of histamine contaminated fish. Good quality fish contains less than 10 ppm histamine. The limit value for fish and products thereof is 100 mg/kg, in some countries even 50 mg/kg. The Swiss limit value for histamine in wine is 10 mg/l.

Specifications
Art. No. R1601 / R1604
Test Format Microtiter plate with 96 or 48 wells (12, R1601 or 6 strips, R1604 with 8 wells each)
Standard Range 0 ppb, 0.5 ppb, 1.5 ppb, 5 ppb, 15 ppb, 50 ppb histamine
Sample Preparation different depending on the sample
Incubation Time 90 min incubation time with shaker: 70 min
Detection Limit 0.1 – 100 mg/kg (ppm) histamine depending on the matrix
sparkling wine, white and red wine: 250 ppb histamine
milk: 100 ppb histamine
cheese, fresh and canned fish: 2.5 ppm histamine
fish meal: 100 ppm histamine
Cross Reactivity Histamine: 100 %3-Methyl-Histamine: ca. 0,01 %Tyramine: n.n.L-Phenylalanine: n.n.

L-Histidine: n.n.

L-Tyrosine: n.n.

Tryptamine: n.n.

5-Hydroxy-Indole-Acetic Acid: n.n.

Serotonine: n.n.

 

Accessories

RIDA® Sample Decolorant

 

 

Intended use:

RIDA® Sample Decolorant (patent pending EP 160 000 276.2) is used for the preparation of samples, such as:

  • wine for RIDASCREEN® Histamine (Enzymatic) (R1605)
  • liver, feed for RIDASCREEN® Clenbuterol (R1711)

Thereby, RIDA® Sample Decolorant removes interfering substances by precipitation. Thereafter the samples can be analyzed according to the respective instructions for use or application note

Specifications
Art. No. R1699
Test Format 1 Set (200 wines samples)

Allergens

Accessories
DNA Preparation
Almond
Brazil nut
Casein
Cashew kernel
Celery
Coconut
Crustaceans
Egg
Fish
Gliadin / Gluten
Hazelnut
Histamine
Lupine
Lysozyme
Macadamia nut
Milk
Molluscs
Mustard
Peanut
Pistachio