Macadamia nut


Lateral Flow Macadamia Nut


Intended use:

The Lateral Flow Macadamia Nut assay is a qualitative detection assay of Macadamia Nut residues in surface samples got from e.g. production lines. The assay is also applicable for food samples. The assay is applied for the detection of small amounts of Macadamia Nut contaminations.

General Information:

Macadamia Nut contaminations in food possibly elicit hypersensitivity in sensitive persons. Food production lines have to be free of residues to prevent these allergic reactions. The effectiveness of cleaning can be verified by using the Lateral Flow Macadamia Nut Swabbing method.

Art. No. BL605-10
Test Format 10 x test strips (1 determination per strip)
25 x test strips (1 determination per strip)
Sample Preparation Swabbing method: cleaning surface with pre-treated swab, wash out the swab and measure
Food assay: homogenise food sample, filter, measure
Incubation Time 10 min
Detection Limit Swabbing method: 10 µg dried sample on surface
Food assay (zero matrix): 1 ppm
Cross Reactivity 0.0001 % Walnut
External Link I



Intended use:

RIDASCREEN®FAST Macadamia is a sandwich enzyme immunoassay for the quantitative analysis of macadamia nut in bakery products, ice cream, cereals, chocolate and other food.

General Information:

Nuts are one of the most common foods causing allergic reactions in children and adults. Allergy to nuts can lead to anaphylaxis symptoms. Also Macadamia nut ingestion can cause immediate-type allergic reactions.
Macadamia nut could occur as ingredient or contamination in raw or processed food. Nuts, also Macadamia nuts, have to be declared if used as food ingredient in Europe, USA, Canada, Australia and New Zealand. In Europe directive 2007/68/EG is applied.
Macadamia nuts are also known by various other names like Bopple nut, Australian nut, Macadamia integrifolia, Macadamia tetraphylla, Bush nut and Queensland nut. They are originating from Australia. The nuts are the seeds of the Macadamia plant which belongs to Proteaceae family.
The best way to avoid nut allergy is to avoid eating nuts in any form. Check label ingredients before buying any product. Candies, baked goods and other foods could contain Macadamia nuts and other nuts as contamination.

Art. No. R6852
Test Format Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Standard Range 0 ppm (zero standard), 1 ppm, 3 ppm, 9 ppm, 27 ppm macadamia nut
Sample Preparation homogenization, extraction and centrifugation
Incubation Time 30 min
Detection Limit 0.17 mg/kg (ppm) almond corresponding to 0.00017 % almond (0.1 – 0.4 depending on the matrix)
Cross Reactivity The antibodies used in the test specifically detect all kinds of macadamia.Low cross reactivity to kidney bean (0.047 %), pinto bean (0.038 %), white bean (0.001 %)



SureFood® ALLERGEN Macademia  – NEW


Intended use:

The test detects macadamia DNA according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control.

For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 containing 40 mg macadamia / kg food sample and a detection system including a standard dilution is required.

For DNA preparation, the use of SureFood® PREP Advanced, protocol 1, is recommended.

General Information:

The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, AriaDx and Agilent.

Art. No. S3616
Test Format Test-Kit with 100* reactions
Sample Preparation 1. DNA preparation, e.g. SureFood® PREP ALLERGEN 2. Preparation of Allergen-PCR mix 3. Pipetting of samples and PCR mix 4. Check PCR protocol settings 5. Start PCR run
Detection Limit Depending on the sample matrix, processing grade, DNA preparation and DNA contant, the detection is limited to < 5 DNA copies / ≤ 0.4 ppm.
Shelf life 12 month
Additional Information The detection system system can be used on all available real-time PCR cyclers.ABI Prism
Eppendorf realplex

*Includes Internal Amplification Control (IAC) in VIC/HEX channel. SureFood®QUANTARD Allergen 40 must be used for quantifiction.


DNA Preparation
Brazil nut
Cashew kernel
Gliadin / Gluten
Macadamia nut