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Milk

 

Lateral Flow Milk

 

Intended use:

The Lateral Flow Milk assay is a qualitative detection assay of Milk residues in surface samples got from e.g. production lines. The assay is also applicable for food samples. The assay is applied for the detection of small amounts of Milk contaminations.

General Information:

Milk contaminations in food possibly elicit hypersensitivity in sensitive persons. Food production lines have to be free of residues to prevent these allergic reactions. The effectiveness of cleaning can be verified by using the Lateral Flow Milk Swabbing method.

Specifications
Art. No. BL613-10
BL613-25
Test Format 10 x test strips (1 determination per strip)
25 x test strips (1 determination per strip)
Sample Preparation Swabbing method: cleaning surface with pre-treated swab, wash out the swab and measure
Food assay: homogenise food sample, filter, measure
Incubation Time 10 min
Detection Limit Swabbing method: 0.2 – 10 µg dried sample on surface
Food assay (zero matrix): 0.1 – 1ppm (depending on the matrix) milk protein
External Link I http://www.bioavid.de/

RIDASCREEN®FAST Milk

 

Intended use:

RIDASCREEN®FAST Milk is a sandwich enzyme immunoassay to quantify milk proteins in food containing whey, milk or milk powder such as sausages, ice cream, chocolate, bakery goods, cake and bread mix, soups, sauces, dressings and beverages (juice, wine, beer). The antibodies specifically detect caseins as well as β-lactoglobulin of cow’s milk and of sheep’s, goat’s and buffalo’s milk.

Approvals:

The ELISA RIDASCREEN®FAST Milk was approved by the AOAC Performance Tested MethodsSM Program and was assigned PTM Certification No. 101501 by the AOAC Research Institute. The in-house validation data was confirmed by an independent lab. The sandwich ELISA is applicable for the detection of milk protein (casein und b-lactoglobulin) in cookies, chocolate, ice cream, infant formula and sausages.

↗ PTM Certification No. 101501

The assay results are quantitative if the sample contains milk or milk powder. If the milk protein composition of the sample is not known and the milk protein ratio is different than the natural ratio in milk (e.g. a sample primarily consisting of whey), the results may be underestimated. If a quantitative result for caseins and/or β-lactoglobulin is requested, the sample should be tested in the RIDASCREEN®FAST Casein (R4612) and/or the RIDASCREEN®FAST β-lactoglobulin (R4902).

RIDASCREEN®FAST Milk has been certified by the AOAC Research Institute as “Performance Tested Method”.

↗ AOAC Performance Tested MethodSM 101501

 

General Information:

Cow`s milk contains 3.2 % proteins which consist of 10 % β-lactoglobulin (leading protein of whey) and 80 % caseins. The most important allergen is β-lactoglobulin especially for children while the caseins become to be dominant later in adults.

Casein (lat. Caseus = cheese) is a rough flaked curdling protein, which forms micells in the milk and precipitates under acidic conditions. The group of caseins consists of the αs-caseins, the β-caseins, the κ-casein, and the γ-caseins (proteolytic protein fragment of β-casein by the milk protease plasmin). Κ-casein is cleaved into a hydrophobic (para κ-casein) and into a water soluble polar component (macropeptid) by proteolysis, e.g. by using lab ferment.
The main components of whey are β-lactoglobulin and α-lactalbumin besides lactoferrin, bovine serum albumin and immunoglobulins.

Milk can be present as an ingredient or as a contamination in raw and processed food products. Because milk can induce allergic reactions at infancy it must be declared as an ingredient on food labels. Whey (β-lactoglobulin) or caseinates (e.g. in sausages) are often added to food products, therefore it is recommended to determine casein in food.

Specifications
Art. No. R4652
Test Format Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Standard Range 0 ppm, 2.5 ppm, 7.5 ppm, 22.5 ppm, 67.5 ppm milk protein
Sample Preparation homogenization, extraction and centrifugation
Incubation Time 30 min
Detection Limit

Limit of quantification

0.7 mg/kg (ppm) milk protein (0.3 – 0.8 depending on the matrix)

 

Limit of quantification: 2.5 mg/kg (ppm) milk protein

Specificity The antibodies specifically detect α-, ß- and κ-caseins as well as ß-lactoglobulin of cow’s milk and of sheep’s, goat’s and buffalo’s milk.

RIDASCREEN® β-Lactoglobulin

 

Intended use:

RIDASCREEN® β-Lactoglobulin is a competitive enzyme immunoassay for the quantitative analysis of β-lactoglobulin in hydrolyzed milk products including hypoallergenic baby food. The assay is calibrated to  hydrolyzed β-lactoglobulin check samples; the standards consist of native β-lactoglobulin. The assay detects native and processed protein and fragments thereof (during the heating of milk, the native ß-lactoglobulin is partially denatured).

Specifications
Art. No. R4901
Test Format Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Standard Range 0 ppm (zero standard), 2.5 ppm, 5 ppm, 10 ppm, 20 ppm almond
Sample Preparation homogenization, extraction and centrifugation
Incubation Time 30 min
Detection Limit 1.4 mg/kg (ppm) β -Lactoglobulin (0.9 – 2.1 depending on the matrix)
Cross Reactivity The polyclonal antibody specifically detects proteins from almonds. Cross reaction to apricot stone is > 100 %. No cross-reaction with cashew nut, brazil nut, pecan, hazelnut, coconut, macadamia nut, walnut, chest nut, sunflower seeds, sesame and lima beans.

RIDASCREEN®FAST β-Lactoglobulin

 

 Intended use:

RIDASCREEN®FAST β-Lactoglobulin (Art. No. R4912) is a sandwich enzyme mmunoassay for the quantitative analysis of native and processed β-lactoglobulin in rice crispies, chocolate, and sausage.

Specifications
Art. No. R4912
Test Format Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Standard Range 0 ppm (zero standard), 2.5 ppm, 5 ppm, 10 ppm, 20 ppm almond
Sample Preparation homogenization, extraction and centrifugation
Incubation Time 30 min
Detection Limit 0.042 mg/kg (ppm) β -Lactoglobulin (0.024 – 0.073 depending on the matrix)
Cross Reactivity The polyclonal antibody specifically detects proteins from almonds. Cross reaction to apricot stone is > 100 %. No cross-reaction with cashew nut, brazil nut, pecan, hazelnut, coconut, macadamia nut, walnut, chest nut, sunflower seeds, sesame and lima beans.

RIDA® Extractor 2

 

Intended use:

The RIDA® Extractor 2 (R4613) is used for the sample preparation in

  • RIDASCREEN®FAST Milk (R4652)
  • RIDASCREEN®FAST Casein (R4612)
  • RIDASCREEN®FAST β-Lactoglobulin (R4902).

The sample preparation is described in the product information of the respective kits.

Specifications
Art. No. R4613
Test Format 30 ml concentrate
sufficient for 15 samples

Reference Material

 

Certified reference material plays a decisive role in food analysis. But so far, suitable material was missing for allergen analysis. Now, MoniQA Association has presented the first validated reference material for the analysis of milk allergen.

For many laboratories, they are an important component of quality assurance: certified reference materials. They allow the user to assess whether the test results are reliable and to rule out false positive or false negative results. Only recently, we emphasized the importance of reference material. However, certified reference material is not available for every field of analysis. One major challenge is the production of reference material for allergen analysis. Allergenic proteins may alter and change their structure or concentrations – and thus cause instability and unconformity.

Allergen analysis is a field with a high demand for certified reference material. The most common technique for the detection of allergens is an ELISA test – a method which is very effective, easy to use and relatively cheap. Yet, comparability of measured results is difficult without reference material. Due to high demand, there have been continuing efforts to develop certified reference material also for allergen analysis. In 2013, the renowned MoniQA Association (International Association for Monitoring and Quality Assurance in the Total Food Supply Chain) launched a respective initiative.

Since January 2017, the first validated reference material for food allergens is available at R-Biopharm. The portfolio, produced by Trilogy Laboratories, includes four testing materials for milk allergen:

 

Milk reference material
SMP-MQA 092014
1 x 5 g
Positive control, 5 g of dried skim milk powder, characterized
BLANK-MQA082015
1 x 5 g
 Negative control: 5 g of gluten free cookie, milled, free from   gluten, milk, egg, soy, peanut, tree nuts
LOW-MQA 102016
1 x 5 g
Incurred material, concentration approx. 10 ppm,
5 g of dried skim milk powder incurred in gluten free cookies, milled, concentration of milk protein is 3.5 mg/kg
HIGH-MQA 082016
1 x 5 g
Incurred material, concentration approx. 50 ppm,
5 g of dried skim milk powder incurred in gluten free cookies, milled, concentration of milk protein is 17.7 mg/kg
SET of 4 MoniQA Reference controls – Blank,SMP,High,Low
4 x 5 g
Set of 4: set includes 5 g pouches of Blank, SMP, High, Low

The samples are available individually and as a set of four (Art. No. MQA 122016). The materials come in 5 g pouches. According to MoniQA Association, further reference materials are in preparation (gluten and soya) and will be available shortly.

 

Allergens

Accessories
DNA Preparation
Almond
Brazil nut
Casein
Cashew kernel
Celery
Coconut
Crustaceans
Egg
Fish
Gliadin / Gluten
Hazelnut
Histamine
Lupine
Lysozyme
Macadamia nut
Milk
Molluscs
Mustard
Peanut
Pistachio