Lateral Flow Pistachio
The Lateral Flow Pistachio assay is used for the qualitative detection of Pistachio residues in surface samples got from e.g. production lines. The assay is also applicable for food samples. The assay is applied for the detection of small amounts of Pistachio contaminations.
Pistachio contaminations in food possibly elicit hypersensitivity in sensitive persons. Food production lines have to be free of residues to prevent these allergic reactions. The effectiveness of cleaning can be verified by using the Lateral Flow Pistachio Swabbing method.
|Test Format||10 x test strips (1 determination per strip)
25 x test strips (1 determination per strip)
|Sample Preparation||Swabbing method: cleaning surface with pre-treated swab, wash out the swab and measure
Food assay: homogenise food sample, filter, measure
|Incubation Time||10 min|
|Detection Limit||Swabbing method: 10 µg dried sample on surface Food assay (zero matrix): 1 ppm|
|Cross Reactivity||Brazil Nut: 0.1 %, Cashew: 4 %, Hazelnut: 0.1 %, Pumpkin seed: 0.1 %, Walnut: 0.8 %|
|External Link I||http://www.bioavid.de/|
SureFood® ALLERGEN Pistachio – NEW
The test detects pistachio DNA according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control.
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 containing 40 mg pistachio / kg food sample and a detection system including a standard dilution is required.
For DNA preparation, the use of SureFood® PREP Advanced, protocol 1, is recommended.
PCR (Polymerase Chain Reaction) amplifies the DNA target sequence, which is subsequently detected via fluorescence labelled hybridization probes in real time.
All SureFood® real time PCR kits use FAM/TAMRA labelled TaqMan probes.
PCR protocols are available for:
ABI PRISM 7000/7700/7900 SDS (Applied Biosystems)
Mx3000P, Mx4000 (Stratagene)
Rotor-Gene (Corbett Research)
Opticon (MJ Research / Oxoid)
Smart Cycler (Cepheid)
Mastercycler ep realplex (Eppendorf)
The PCR kit can be performed all commonly used real-time PCR Cyclers
|Test Format||Test-Kit with 100* reactions|
|Sample Preparation||1. DNA preparation (SureFood® PREP Allergen, S1012) 2. PCR set up 3. PCR run|
|Detection Limit||≤ 5 DNA copies / ≤ 0.4 ppm depending on the sample matrix, processing grade, DNA preparation and DNA content|
|Cross Reactivity||none known|
|Shelf life||12 month|
|Additional Information||Materials required but not provided with the test kit:• Reagents for DNA preparation (SureFood® Prep)
• real-time PCR instrument
• Optical tubes and caps / LightCycler capillaries
• Pipette tips with filter (Filter tips) to reduce risk of contamination
*Includes Internal Amplification Control (IAC) in VIC/HEX channel. SureFood®QUANTARD Allergen 40 must be used for quantifiction.
Gliadin / Gluten